An Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy

Authors

Shigao Huang, Department of Radiation Oncology, Xijing Hospital, Air Force Medical University, Xi'an, China.
Cheng Jiang, Nuffield Department of Clinical Neurosciences and Kavli Institute for Nanoscience Discovery, New Biochemistry Building, University of Oxford, Oxford, United Kingdom.
Muhammad Jameel Mughal, Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, The George Washington University, Columbia, WA, United States.
Guanyu Wang, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
Fuqiang Xing, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.

Document Type

Journal Article

Publication Date

1-1-2022

Journal

Frontiers in bioengineering and biotechnology

Volume

10

DOI

10.3389/fbioe.2022.839078

Keywords

FRET technique; apoptosis; cancer therapy; drug discovery; xenograft model

Abstract

In the present study, an efficient drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the drug-induced cell apoptosis. Furthermore, the C3 expressing cancer cells were then injected into zebrafish embryos and nude mice to establish the corresponding xenograft models. We found that cancer cell lines expressing C3 were effective in detecting cell death following drug treatment, including the detection of the tipping point of apoptosis. The drug-induced cell apoptosis was also observed in both zebrafish embryos and nude mice xenograft models. Overall, the FRET-based platform, through imaging, is potentially useful to improve drug screening efficiency.

APA Citation

Huang, Shigao; Jiang, Cheng; Mughal, Muhammad Jameel; Wang, Guanyu; and Xing, Fuqiang, "An Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy" (2022). GW Authored Works. Paper 189.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/189

Department

Biochemistry and Molecular Medicine