Clostridia isolated from helminth-colonized humans promote the life cycle of Trichuris species

Shushan Sargsian, Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Ze Chen, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Soo Ching Lee, Type 2 Immunity Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Amicha Robertson, Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Rafaela Saes Thur, Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.
Julia Sproch, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Joseph C. Devlin, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Mian Zi Tee, Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Yi Xian Er, Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Richard Copin, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Adriana Heguy, Genome Technology Center, Office of Science and Research, New York University Langone Health, New York, NY 10016, USA; Department of Pathology, New York University Grossman School of Medicine, New York, NY 10016, USA.
Alejandro Pironti, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY 10016, USA; Microbial Computational Genomic Core Lab, New York University Grossman School of Medicine, New York, NY 10016, USA.
Victor J. Torres, Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Antimicrobial-Resistant Pathogens Program, New York University Grossman School of Medicine, New York, NY 10016, USA.
Kelly V. Ruggles, Institute for System Genetics, New York University Langone Health, New York, NY 10016, USA; Division of Precision Medicine, Department of Medicine, New York University Langone Health, New York, NY 10016, USA.
Yvonne A. Lim, Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Jeffrey Bethony, Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.
P'ng Loke, Type 2 Immunity Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: png.loke@nih.gov.
Ken Cadwell, Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Division of Gastroenterology and Hepatology, Department of Medicine, New York University Langone Health, New York, NY 10016, USA. Electronic address: ken.cadwell@nyulangone.org.

Document Type

Journal Article

Publication Date

11-29-2022

Journal

Cell reports

Volume

41

Issue

9

DOI

10.1016/j.celrep.2022.111725

Keywords

CP: Microbiology; Clostridia; Trichuris; albendazole; helminth; indigenous population; metagenomic sequencing; microbiome

Abstract

Soil-transmitted intestinal worms known as helminths colonize over 1.5 billion people worldwide. Although helminth colonization has been associated with altered composition of the gut microbiota, such as increases in Clostridia, individual species have not been isolated and characterized. Here, we isolate and sequence the genome of 13 Clostridia from the Orang Asli, an indigenous population in Malaysia with a high prevalence of helminth infections. Metagenomic analysis of 650 fecal samples from urban and rural Malaysians confirm the prevalence of species corresponding to these isolates and reveal a specific association between Peptostreptococcaceae family members and helminth colonization. Remarkably, Peptostreptococcaceae isolated from the Orang Asli display superior capacity to promote the life cycle of whipworm species, including hatching of eggs from Trichuris muris and Trichuris trichiura. These findings support a model in which helminths select for gut colonization of microbes that support their life cycle.

Department

Microbiology, Immunology, and Tropical Medicine

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