Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon

Madeleine Eunice Betouke Ongwe, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Yoanne D. Mouwenda, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Koen A. Stam, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Peter G. Kremsner, Institut für Tropenmedizin, Eberhad Karls Universität Tübingen, Tübingen, Germany.
Bertrand Lell, Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.
David Diemert, George Washington University, Washington, DC, United States of America.
Jeff Bethony, George Washington University, Washington, DC, United States of America.
Maria E. Bottazzi, Texas Children's Hospital Center for Vaccine Development, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, United States of America.
Peter J. Hotez, Texas Children's Hospital Center for Vaccine Development, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, United States of America.
Remko V. Leeuwen, Amsterdam Institute for Global Health and Development, Amsterdam, The Netherlands.
Martin P. Grobusch, Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.
Ayola A. Adegnika, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Oleg A. Mayboroda, Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Maria Yazdanbakhsh, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.

Document Type

Journal Article

Publication Date

1-1-2022

Journal

PloS one

Volume

17

Issue

9

DOI

10.1371/journal.pone.0275013

Abstract

Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity.

Department

Medicine

Link to Full Text

Share

COinS