Title

Gel filtration in 6 M guanidine hydrochloride of the α-subunit (and its fragments) of the receptor for immunoglobulin E

Document Type

Journal Article

Publication Date

1-1-1982

Journal

Molecular Immunology

Volume

19

Issue

12

DOI

10.1016/0161-5890(82)90267-X

Abstract

The mol. wts of the α-chain of the receptor for immunoglobulin E and several of its enzyme-cleaved fragments have been evaluated by gel filtration on Sepharose 6B in 6 M guanidine HCl. The mol. wt of α-chains treated with endoglycosidase was 30% less than that of untreated α-chains. α-Chains digested with papain eluted in a single peak with a mol. wt approximately one-half of that of undigested α-chains. The results support the proposal that papain cleaves α-chains into two fragments of similar size [Goetze et al. (1981) Biochemistry 20, 6341-6349]. © 1982.

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