Mouse bladder wall injection

Document Type

Journal Article

Publication Date

1-1-2011

Journal

Journal of Visualized Experiments

Issue

53

DOI

10.3791/2523

Keywords

Bladder; Bladder cancer; Bladder wall injection; Intramural injection; Issue 53; Medicine; Stem cell

Abstract

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. Before starting injections, the surgical area must be cleaned with soap and water and antiseptic solution. Surgical equipment must be sterilized before use and between each animal. Each mouse is placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and its bladder exposed by making a midline abdominal incision with scissors. If the bladder is full, it is partially decompressed by gentle squeezing between two fingers. The cell suspension of interest is intramurally injected into the wall of the bladder dome using a 29 or 30 gauge needle and 1 cc or smaller syringe. The wound is then closed using wound clips and the mouse allowed to recover on a warming pad. Bladder wall injection is a delicate microsurgical technique that can be mastered with practice. © 2011 Journal of Visualized Experiments.

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