Preparation of radiopaque drug-eluting beads for transcatheter chemoembolization
Journal of Vascular and Interventional Radiology
© 2016 SIR. Purpose To develop a simple method to produce radiopaque drug-eluting microspheres (drug-eluting beads [DEBs]) that could be incorporated into the current clinical transcatheter arterial chemoembolization workflow and evaluate their performance in vitro and in vivo. Materials and Methods An ethiodized oil (Lipiodol; Guerbet, Villepinte, France) and ethanol solution was added to a lyophilized 100-300 μm bead before loading with doxorubicin. These radiopaque drug-eluting beads (DEBs; Biocompatibles UK Ltd, Farnham, United Kingdom) were evaluated in vitro for x-ray attenuation, composition, size, drug loading and elution, and correlation between attenuation and doxorubicin concentration. In vivo conspicuity was evaluated in a VX2 tumor model. Results Lipiodol was loaded into lyophilized beads using two glass syringes and a three-way stopcock. Maximum bead attenuation was achieved within 30 minutes. X-ray attenuation of radiopaque beads increased linearly (21-867 HU) with the amount of beads (0.4-12.5 vol%; R2 = 0.9989). Doxorubicin loading efficiency and total amount eluted were similar to DC Bead (Biocompatibles UK Ltd); however, the elution rate was slower for radiopaque DEBs (P <.05). Doxorubicin concentration linearly correlated with x-ray attenuation of radiopaque DEBs (R2 = 0. 99). Radiopaque DEBs were seen in tumor feeding arteries after administration by fluoroscopy, computed tomography, and micro-computed tomography, and their location was confirmed by histology. Conclusions A simple, rapid method to produce radiopaque DEBs was developed. These radiopaque DEBs provided sufficient conspicuity to be visualized with x-ray imaging techniques.
Johnson, C., Tang, Y., Beck, A., Dreher, M., Woods, D., Negussie, A., Donahue, D., Levy, E., Willis, S., Lewis, A., Wood, B., & Sharma, K. (2016). Preparation of radiopaque drug-eluting beads for transcatheter chemoembolization. Journal of Vascular and Interventional Radiology, 27 (1). http://dx.doi.org/10.1016/j.jvir.2015.09.011