High-speed fiber-optic scanning nonlinear endomicroscopy for imaging neuron dynamics in vivo
Fiber-optic-based two-photon fluorescence endomicroscopy is emerging as an enabling technology for in vivo histological imaging of internal organs and functional neuronal imaging on freely-behaving animals. However, high-speed imaging remains challenging due to the expense of miniaturization and lack of suited fast beam scanners. For many applications, a higher imaging speed is highly desired, especially for monitoring functional dynamics such as transient dendritic responses in neuroscience. This Letter reports the development of a fast fiber-optic scanning endomicroscope with an imaging speed higher than 26 frames/s. In vivo neural dynamics imaging with the high-speed endomicroscope was performed on a freely-behaving mouse over the primary motor cortex that expressed GCaMP6m. The results demonstrate its capability of real-time monitoring of transient neuronal dynamics with very fine temporal resolution.
Park, H., Guan, H., Li, A., Yue, Y., Li, M., Lu, H., & Li, X. (2020). High-speed fiber-optic scanning nonlinear endomicroscopy for imaging neuron dynamics in vivo. Optics Letters, 45 (13). http://dx.doi.org/10.1364/OL.396023