Channel gating regulation by the cystic fibrosis transmembrane conductance regulator (CFTR) first cytosolic loop
Journal of Biological Chemistry
Background: Elucidating the mechanochemistry of ABC transporters is essential for understanding several human disease states. Results: We show a novel intermolecular binding step that governs CFTR channel activity. Conclusion: CFTR gating and ATP hydrolysis require a properly configured transmembrane domain interface. Significance: These findings indicate a new approach for discovering therapeutic targets within ABC proteins that modulate function.
Ehrhardt, A., Chung, W., Pyle, L., Wang, W., Nowotarski, K., Mulvihill, C., Ramjeesingh, M., Hong, J., Velu, S., Lewis, H., Atwell, S., Aller, S., Bear, C., Lukacs, G., Kirk, K., & Sorscher, E. (2016). Channel gating regulation by the cystic fibrosis transmembrane conductance regulator (CFTR) first cytosolic loop. Journal of Biological Chemistry, 291 (). http://dx.doi.org/10.1074/jbc.M115.704809