Title

Role of PECAM-1 (CD31) in neutrophil transmigration in murine models of liver and peritoneal inflammation

Document Type

Journal Article

Publication Date

1-1-1998

Journal

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volume

274

Issue

4 37-4

DOI

10.1152/ajpgi.1998.274.4.g776

Keywords

Adhesion molecules; Endotoxin; Integrins; Peritonitis; Sepsis

Abstract

Platelet endothelial cell adhesion molecule-1 (PECAM-1) is thought to be critical for transendothelial migration of leukocytes, including neutrophils. Because neutrophil-mediated liver injury during endotoxemia is dependent on transmigration, we investigated the role of PECAM-1 in the pathophysiology of endotoxin-induced liver injury. Male C3Heb/FeJ mice were treated with galactosamine (Gal) and endotoxin (ET) (700 mg/kg Gal/100 μg/kg ET), and liver sections were stained for PECAM-1 expression. Control livers showed the presence of PECAM-1 on endothelial cells of large vessels but not in sinusoids. Gal/ET treatment did not change the expression pattern of PECAM- 1. Gal/ET-induced liver injury (area of necrosis: 38 ± 3%) was not attenuated by treatment with 3 mg/kg of the antimurine PECAM-1 antibody 2H8. The antibody had no effect on sequestration and transmigration of neutrophils in sinusoids or the margination of neutrophils in large vessels. In contrast, 2H8 inhibited glycogen-induced neutrophil migration into the peritoneum by 74%; this effect correlated with PECAM-1 expression in the intestinal vasculature. Thus PECAM-1 is neither expressed nor inducible in hepatic sinusoids and is consequently not involved in neutrophil transmigration in the liver during endotoxemia. On the other hand, expression of PE-CAM-1 in mesenteric veins is critical for peritoneal neutrophil accumulation.

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