Title

Thyroid transcription factor-1 immunocytochemical staining of pleural fluid cytocentrifuge preparations for detection of small cell lung carcinoma

Document Type

Journal Article

Publication Date

1-1-2004

Journal

Acta Cytologica

Volume

48

Issue

5

DOI

10.1159/000326434

Keywords

Carcinoma, small cell lung; Immunocytochemistry; Pleural effusion; Thyroid transcription factor-1; Transcription factors

Abstract

OBJECTIVE: To assess whether thyroid transcription factor-1(TTF-1) immunostaining of cytocentrifuge slides previously stained by the Papanicolaou technique is feasible and useful in diagnosing small cell lung carcinoma (SCLC) in pleural fluid. STUDY DESIGN: Eleven Papanicoalou-stained cytocentrifuge preparations of pleural fluid from 11 patients with SCLC were reviewed. The initial cytologic diagnoses were 5 positive, 1 suspicious and 5 negative for SCLC. After transferring the cytologic material to a positively charged slide, immunostaining was performed using mouse monoclonal antibody against TTF-1. RESULTS: The neoplastic cellularity varied among the single suspicious and 5 positive cases. The case with a suspicious diagnosis had scant tumor cells. All 6 cases showed nuclear staining for TTF-1 in most of the tumor cells. The other 5 cases with a negative cytologic diagnosis were negative for TTF-1. CONCLUSION: The results of this study indicate that immunostaining for TTF-1 can be successfully applied to previously Pap-stained cytocentrifuge preparations to assist in the diagnosis of SCLC in pleural fluid.

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