Selective and reversible increase in the number of quisqualate-sensitive glutamate binding sites on hippocampal synaptic membranes after angular bundle kindling
epilepsy; glutamate; hippocampus; kindling; neurotransmitter receptors
The specific binding of l-[3H]glutamate to hippocampal synaptic membranes was examined in rats kindled by tetanic stimulation of the angular bundle. One day after the last of a minimum of 3 class 4 kindled seizures, the binding of l-[3H]glutamate to a quisqualate-sensitive site (GLU A) was about 40% greater than in electrode-implanted unstimulated controls. Saturation binding data indicated an increase in the maximum density of GLU A binding sites with no change in their affinity for l-glutamate. No such increase was detected 28 days after the last kindled seizure, although the animals were still kindled. Radioligand binding to a site that is much less sensitive to quisqualate (GLU B) was unaffected by kindling stimulation. These observations suggest that an increase in GLU A binding sites could be involved in the induction, but not in the maintenance, of the kindled state. © 1984.
Savage, D., Werling, L., Nadler, J., & McNamara, J. (1984). Selective and reversible increase in the number of quisqualate-sensitive glutamate binding sites on hippocampal synaptic membranes after angular bundle kindling. Brain Research, 307 (1-2). http://dx.doi.org/10.1016/0006-8993(84)90488-8