SH-SY5Y cells as a model for sigma receptor regulation of potassium- stimulated dopamine release
(+)-Pentazocine; Dopamine; NPY; SH-SY5Y; Sigma receptor
Previous studies in our laboratory using rat brain tissue have shown that neuropeptide Y (NPY) can enhance NMDA- and potassium-stimulated dopamine release from various brain regions and that this enhancement is reversed by sigma (σ) receptor antagonists. In the current study, we sought to determine whether SH-SY5Y cells are suitable for investigating σ receptor effects and whether any σ receptors present are of the subtype responsive to NPY. We compare mechanisms by which the prototypical σ receptor agonist (+)- pentazocine, and the proposed endogenous σ receptor ligand NPY regulate potassium-stimulated [3 H]dopamine release from SH-SY5Y cells. Both (+)- pentazocine and NPY inhibit potassium-stimulated [3 H]dopamine release. Unlike our studies in rat brain tissue, the effect of NPY on [3 H]dopamine release is not reversed by σ receptor antagonists. SH-SY5Y cells appear to be an appropriate model to study the regulation of dopamine release by σ receptors or by NPY receptors, but this population is not identical to that population identified in brain slices. (C) 2000 Elsevier Science B.V.
Ault, D., & Werling, L. (2000). SH-SY5Y cells as a model for sigma receptor regulation of potassium- stimulated dopamine release. Brain Research, 877 (2). http://dx.doi.org/10.1016/S0006-8993(00)02722-0