Title

SH-SY5Y cells as a model for sigma receptor regulation of potassium- stimulated dopamine release

Document Type

Journal Article

Publication Date

9-22-2000

Journal

Brain Research

Volume

877

Issue

2

DOI

10.1016/S0006-8993(00)02722-0

Keywords

(+)-Pentazocine; Dopamine; NPY; SH-SY5Y; Sigma receptor

Abstract

Previous studies in our laboratory using rat brain tissue have shown that neuropeptide Y (NPY) can enhance NMDA- and potassium-stimulated dopamine release from various brain regions and that this enhancement is reversed by sigma (σ) receptor antagonists. In the current study, we sought to determine whether SH-SY5Y cells are suitable for investigating σ receptor effects and whether any σ receptors present are of the subtype responsive to NPY. We compare mechanisms by which the prototypical σ receptor agonist (+)- pentazocine, and the proposed endogenous σ receptor ligand NPY regulate potassium-stimulated [3 H]dopamine release from SH-SY5Y cells. Both (+)- pentazocine and NPY inhibit potassium-stimulated [3 H]dopamine release. Unlike our studies in rat brain tissue, the effect of NPY on [3 H]dopamine release is not reversed by σ receptor antagonists. SH-SY5Y cells appear to be an appropriate model to study the regulation of dopamine release by σ receptors or by NPY receptors, but this population is not identical to that population identified in brain slices. (C) 2000 Elsevier Science B.V.

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