Agarose slurry as a support medium for bioprinting and culturing freestanding cell-laden hydrogel constructs
3D Printing and Additive Manufacturing
agarose slurry; cell-laden bioinks; hydrogels
© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019. We present a modified method of embedded bioprinting, which allows maintaining freestanding three-dimensional (3D) printed structures in cell culture conditions for extended periods of time. This method, termed CLASS (constructs laid in agarose slurry suspension), was tested using cell-laden alginate and gelatin methacrylate (GelMa)-based bioinks. A direct comparison of 3D printed constructs, supported by gelatin and agarose hydrogel slurries, revealed several advantages, including slurry stability across different print temperatures and blending times, increased slurry homogeneity, and the ability of CLASS to support freestanding constructs for an extended time in cell culture. We conclude that CLASS is a straightforward and cost-efficient way to print and support freestanding cell-laden biomaterials.
Mirdamadi, E., Muselimyan, N., Koti, P., Asfour, H., & Sarvazyan, N. (2019). Agarose slurry as a support medium for bioprinting and culturing freestanding cell-laden hydrogel constructs. 3D Printing and Additive Manufacturing, 6 (3). http://dx.doi.org/10.1089/3dp.2018.0175