TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus
Biochemical and Biophysical Research Communications
Food intake; Hypothalamus; Neuropeptide Y; Obesity; Proopiomelanocortin; TTF-1
TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus. © 2006 Elsevier Inc. All rights reserved.
Kim, J., Nam-Goong, I., Yun, C., Jeong, J., Kim, E., Park, J., Lee, Y., Kim, Y., & Lee, B. (2006). TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus. Biochemical and Biophysical Research Communications, 349 (3). http://dx.doi.org/10.1016/j.bbrc.2006.08.147