Kinetics and mechanism of substitution reactions between myoglobin and activated self-assembled monolayers investigated using surface plasmon resonance
The rate of the reaction between solution-phase myoglobin and SAMs fabricated from w-X-hexadecylthiomethylbenzenes where X was a mesylate, chloride, or bromide was investigated using surface plasmon resonance (spr). The data were fit to a model in which nonspecific adsorption competes with a second-order substitution reaction between myoglobin and the surface species. The second-order reaction rate constants obtained from the regression analysis were 440 ± 140 M-1s-1 for the bromide, 56 ± 10 M-1s-1 for the mesylate, and 38 ± 12 M-1s-1 for the chloride. The experimental rate law fit to the data and the increase in rate with decreased basicity of the leaving group were consistent with an SN2 mechanism.
Deckert, A., Lesko, J., Todaro, S., Doyle, M., & Delaney, C. (2002). Kinetics and mechanism of substitution reactions between myoglobin and activated self-assembled monolayers investigated using surface plasmon resonance. Langmuir, 18 (21). http://dx.doi.org/10.1021/la025767e