Quantitative analysis of metabolic profile and Tamm-Horsfall protein in pediatric stone patients
AIP Conference Proceedings
Soluble calcium; Tamm-Horsfall protein; Urine; Urolithiasis; Uromodulin
According to some studies, the secretion of urinary glycoprotein Tamm-Horsfall protein (uromodulin) plays a significant part in the suppression of calcium nephrolith formation. The aim of the present study was to detect correlation of Tamm-Horsfall protein with soluble Ca2+ in the urine of urolithic patients and compare it with a control group, in order to estimate the degree of the disease progress, as well as the prognosis for the disease. The research included 29 urolithic patients, aged 8,0±0,2 y. The content of soluble Ca2+ in fresh urine was estimated by the method of capillary zone electrophoresis. The level of Tamm-Horsfall protein in urine was measured from cryoprecipitate using SDS Polyacrylamide electrophoresis and semiquantitative analysis. Urine from the urolithic patients was consistently higher in Ca2+ and in Tamm-Horsfall protein concentrations than urine from controls, at all periods of the day. © 2008 American Institute of Physics.
Kogan, M., Matishov, D., Shin, E., & Sizonov, V. (2008). Quantitative analysis of metabolic profile and Tamm-Horsfall protein in pediatric stone patients. AIP Conference Proceedings, 1049 (). http://dx.doi.org/10.1063/1.2998047