Performance of the TruGene human immunodeficiency virus type 1 genotyping kit and OpenGene DNA sequencing system on clinical samples diluted to approximately 100 copies per milliliter

Authors

Howard B. Gale, Washington DC VA Medical Center
Virginia L. Kan, Washington DC VA Medical Center
Rebecca C. Shinol, Washington DC VA Medical Center

Document Type

Journal Article

Publication Date

2-1-2006

Journal

Clinical and Vaccine Immunology

Volume

13

Issue

2

DOI

10.1128/CVI.13.2.235-238.2006

Abstract

The TruGene human immunodeficiency virus type 1 (HIV-1) genotyping kit/OpenGene DNA sequencing system (Bayer HealthCare, Tarrytown, NY) reliably produced clinically acceptable resistance profiles for reverse transcriptase and protease inhibitors on patient samples diluted to ∼100 copies/ml following extraction with the QIAamp viral RNA minikit (QIAGEN Inc., Valencia, CA). One modification of the standard protocol was made to guarantee PCR amplification: a centrifugation step to concentrate virus was added before RNA extraction. For genotypic antiretroviral resistance testing, no significant differences in the identification and sensitivity of detection for codon mutations, base mutations, and multibase sites were found between the original and diluted samples.

APA Citation

Gale, H., Kan, V., & Shinol, R. (2006). Performance of the TruGene human immunodeficiency virus type 1 genotyping kit and OpenGene DNA sequencing system on clinical samples diluted to approximately 100 copies per milliliter. Clinical and Vaccine Immunology, 13 (2). http://dx.doi.org/10.1128/CVI.13.2.235-238.2006