Rat strain effects of AT1 receptor activation on D1 dopamine receptors in immortalized renal proximal tubule cells
Dopamine; Kidney; Rats, spontaneously hypertensive; Receptors, angiotensin II
The dopaminergic and renin-angiotensin systems regulate blood pressure, in part, by affecting sodium transport in renal proximal tubules (RPTs). We have reported that activation of a D1-like receptor decreases AT 1 receptor expression in the mouse kidney and in immortalized RPT cells from Wistar-Kyoto (WKY) rats. The current studies were designed to test the hypothesis that activation of the AT1 receptor can also regulate the D1 receptor in RPT cells, and this regulation is aberrant in spontaneously hypertensive rats (SHRs). Long-term (24 hours) stimulation of RPT cells with angiotensin II, via AT1 receptors increased total cellular D1 receptor protein in a time- and concentration-dependent manner in WKY but not in SHR cells. Short-term stimulation (15 minutes) with angiotensin II did not affect total cellular D1 receptor protein in either rat strain. However, in the short-term experiments, angiotensin II decreased cell surface membrane D1 receptor protein in WKY but not in SHR cells. D1 and AT1 receptors colocalized (confocal microscopy) and their coimmunoprecipitation was greater in WKY than in SHRs. However, AT 1/D1 receptor coimmunoprecipitation was decreased by angiotensin II (10-8M/24 hours) to a similar extent in WKY (-22±8%) and SHRs (-22±12%). In summary, these studies show that AT1 and D1 receptors interact differently in RPT cells from WKY and SHRs. It is possible that an angiotensin II-mediated increase in D1 receptors and dissociation of AT1 from D1 receptors serve to counter regulate the long-term action of angiotensin II in WKY rats; different effects are seen in SHRs. © 2005 American Heart Association, Inc.
Zeng, C., Wang, Z., Hopfer, U., Asico, L., Eisner, G., Felder, R., & Jose, P. (2005). Rat strain effects of AT1 receptor activation on D1 dopamine receptors in immortalized renal proximal tubule cells. Hypertension, 46 (4). http://dx.doi.org/10.1161/01.HYP.0000184251.01159.72