Title

A long-read RNA-seq approach to identify novel transcripts of very large genes.

Document Type

Journal Article

Publication Date

7-6-2020

Journal

Genome research

DOI

10.1101/gr.259903.119

Abstract

RNA-seq is widely used for studying gene expression, but commonly used sequencing platforms produce short reads that only span up to two exon junctions per read. This makes it difficult to accurately determine the composition and phasing of exons within transcripts. Although long-read sequencing improves this issue, it is not amenable to precise quantitation, which limits its utility for differential expression studies. We used long-read isoform sequencing combined with a novel analysis approach to compare alternative splicing of large, repetitive structural genes in muscles. Analysis of muscle structural genes that produce medium (

Comments

This is an open access PubMed Central article.

Peer Reviewed

1

Open Access

1

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