Flow cytometry of fluorescent proteins

William G. Telford
Teresa Hawley
Fedor Subach
Vladislav Verkusha
Robert G. Hawley, George Washington University


Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.