Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging.

Authors

Qinsi Zheng
Anthony X Ayala
Inhee Chung, George Washington University
Aubrey V Weigel
Anand Ranjan
Natalie Falco
Jonathan B Grimm
Ariana N Tkachuk
Carl Wu
Jennifer Lippincott-Schwartz
Robert H Singer
Luke D Lavis

Document Type

Journal Article

Publication Date

9-25-2019

Journal

ACS Cent Sci

Volume

5

Issue

9

DOI

10.1021/acscentsci.9b00676

Abstract

Rhodamine dyes exist in equilibrium between a fluorescent zwitterion and a nonfluorescent lactone. Tuning this equilibrium toward the nonfluorescent lactone form can improve cell-permeability and allow creation of "fluorogenic" compounds-ligands that shift to the fluorescent zwitterion upon binding a biomolecular target. An archetype fluorogenic dye is the far-red tetramethyl-Si-rhodamine (SiR), which has been used to create exceptionally useful labels for advanced microscopy. Here, we develop a quantitative framework for the development of new fluorogenic dyes, determining that the lactone-zwitterion equilibrium constant (

Comments

This is an open access PubMed Central article.

APA Citation

Zheng, Q., Ayala, A., Chung, I., Weigel, A., Ranjan, A., Falco, N., Grimm, J., Tkachuk, A., Wu, C., Lippincott-Schwartz, J., Singer, R., & Lavis, L. (2019). Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging.. ACS Cent Sci, 5 (9). http://dx.doi.org/10.1021/acscentsci.9b00676

Peer Reviewed

1

Open Access

1