CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths

Authors

Akito Koike, Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, D.C. 20037, USA.
Paul J. Brindley, Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, D.C. 20037, USA. Electronic address: pbrindley@gwu.edu.

Document Type

Journal Article

Publication Date

5-19-2025

Journal

International journal for parasitology

DOI

10.1016/j.ijpara.2025.05.001

Keywords

CRISPR; Cas; Genome editing; Helminth parasites; Transgene

Abstract

Functional genomics using CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated endonuclease)-based approaches has revolutionized biomedical sciences. Gene editing is also widespread in parasitology generally and its use is increasing in studies on helminths including flatworm and roundworm parasites. Here, we survey the progress, specifically with experimental CRISPR-facilitated functional genomics to investigate helminth biology and pathogenesis, and also with the burgeoning use of CRISPR-based methods to assist in diagnosis of helminth infections. We also provide an historical timeline of the introduction and uses of CRISPR in helminth species to date.

APA Citation

Koike, Akito and Brindley, Paul J., "CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths" (2025). GW Authored Works. Paper 7244.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/7244

Department

Microbiology, Immunology, and Tropical Medicine