Safety and immunogenicity of an adjuvanted chikungunya virus virus-like particle (CHIKV VLP) vaccine in previous recipients of other alphavirus vaccines versus alphavirus vaccine-naive controls: an open-label, parallel-group, age-matched, sex-matched, phase 2 randomised controlled study

Melinda J. Hamer, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, USA; Department of Emergency Medicine, George Washington University School of Medicine and Health Sciences, Washington, DC, USA.
James M. McCarty, Stanford University School of Medicine, Stanford, CA, USA.
Benjamin C. Pierson, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Jason A. Regules, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA; Uniformed Services University (USU) F Edward Hebert School of Medicine, Bethesda, MD, USA.
Jason Mendy, Bavarian Nordic, San Diego, CA, USA.
Aaron D. Sanborn, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, USA.
Christina L. Gardner, Virology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Jeannine M. Haller, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Melissa K. Gregory, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Dani L. Liggett, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Pamela J. Glass, Virology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Neha Ghosh, Emergent BioSolutions, Gaithersburg, MD, USA.
Sarah Royalty Tredo, Emergent BioSolutions, Gaithersburg, MD, USA.
Kelly L. Warfield, Emergent BioSolutions, Gaithersburg, MD, USA.
Crystal W. Burke, Virology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA.
Christine Lee, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, USA.
David Saunders, Division of Medicine, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, USA; Uniformed Services University (USU) F Edward Hebert School of Medicine, Bethesda, MD, USA.
Lisa Bedell, Bavarian Nordic, San Diego, CA, USA.
Jason S. Richardson, Bavarian Nordic Canada, Toronto, ON, Canada. Electronic address: JARI@bavarian-nordic.com.

Document Type

Journal Article

Publication Date

2-12-2025

Journal

The Lancet. Microbe

DOI

10.1016/j.lanmic.2024.101000

Abstract

BACKGROUND: Immune responses to alphavirus vaccines might be impaired when heterologous alphavirus vaccines are administered sequentially. We aimed to compare immunogenicity and safety of a chikungunya virus virus-like particle (CHIKV VLP) vaccine in previous recipients of heterologous alphavirus vaccines with alphavirus-naive controls in the USA. METHODS: In this open-label, parallel-group, age-matched, sex-matched, phase 2 randomised controlled trial, which was conducted at two clinical study sites in the USA, adults (aged 18-65 years) who had previously received an investigational Venezuelan equine encephalitis virus vaccine (previous alphavirus vaccine recipients; n=30) and sex-matched and age-matched alphavirus vaccine-naive controls (n=30) were intramuscularly administered one 40 μg dose of CHIKV VLP vaccine on day 1. Immunogenicity was based on serum neutralising antibodies assessed by an in-vitro luciferase-based anti-CHIKV NT neutralisation assay. The primary immunogenicity endpoint, which was assessed in the immunogenicity evaluable population (CHIKV VLP-vaccinated participants who had no important protocol deviations, had not received a prohibited medication, and provided evaluable serum sample results for baseline and on day 22), was to compare the proportion of previous alphavirus vaccine recipients with the proportion of alphavirus vaccine-naive controls who reached seroconversion 21 days after vaccination (ie, study day 22) with a single dose of CHIKV VLP vaccine, based on a four-fold increase of CHIKV neutralising antibodies compared with baseline. The significance of the comparison of the two groups was assessed using Fisher's exact test. The proportion with seroconversion in each group is presented with 95% CIs calculated using the Wilson method. The difference and 95% CIs for this difference was calculated based on Newcombe hybrid score method. An ANOVA model was fit with log-transformed titre as the dependent variable, and study arm, age, and sex as predictors. Least squares means, difference, and 95% CIs were back-transformed and reported as geometric mean titres (GMTs). This trial is registered with ClinicalTrials.gov, NCT03992872. FINDINGS: Between Nov 20, 2019, and Jan 19, 2021, 60 participants (20 [33%] female and 40 [67%] male; 40 (67%) White; median age 47·0 years [IQR 13·5]), 30 previous alphavirus vaccine recipients and 30 alphavirus vaccine-naive controls, were enrolled, vaccinated with CHIKV VLP, and completed the trial. The anti-CHIKV neutralising antibody seroconversion rate at day 22 was 100% (95% CI 88·6-100) in both groups. GMTs peaked in previous alphavirus vaccine recipients and alphavirus vaccine-naive controls at day 22 (2032·5 [95% CI 1413·0-2923·6] and 2299·2 [1598·1-3307·8], respectively) and were similar between the groups on day 22 and all subsequent visits. A higher proportion of previous alphavirus vaccine recipients (93·3% [95% CI 78·7-98·2]) had a four-fold neutralising antibody increase at day 8 than did alphavirus vaccine-naive controls (66·7% [48·8-80·8]; p=0·021). There was no statistically significant difference in the incidence of solicited adverse events between the previous alphavirus vaccine recipients and alphavirus vaccine-naive controls (53·3% vs 40·0%, respectively), although the relatively small sample size of the trial limited the power to detect a significant difference, and there were no reported vaccine-related serious adverse events. INTERPRETATION: CHIKV VLP vaccine was well tolerated and similarly immunogenic in both alphavirus vaccine-naive participants and previous recipients of a heterologous alphavirus vaccine. There were no significant differences in adverse events between the groups. The results of this study support the use of CHIKV VLP vaccine in individuals with previous alphavirus vaccine exposure. FUNDING: Defense Health Program, Emergent Travel Health, and Bavarian Nordic A/S.

Department

Emergency Medicine

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