Individualized use of 6-mercaptopurine in Chinese children with ALL: A multicenter randomized controlled trial
Clinical pharmacology and therapeutics
Continuous 6-mercaptopurine (6-MP) dose titration is necessary because of its narrow therapeutic index and frequently encountered dose-limiting hematopoietic toxicity. However, evidence-based guidelines for gene-based 6-MP dosing have not been established for Chinese children with acute lymphoblastic leukemia (ALL). This multicenter, randomized, open-label, active-controlled clinical trial randomly assigned Chinese children with low- or intermediate-risk ALL in a 1:1 ratio to receive TPMT-NUDT15 gene-based dosing of 6-MP (N = 44, 10 to 50 mg/m /day) or standard dosing (N = 44, 50 mg/m /day) during maintenance therapy. The primary endpoint was the incidence of 6-MP myelosuppression in both groups. Secondary endpoints included frequencies of 6-MP hepatotoxicity, duration of myelosuppression and leukopenia, event-free survival, and steady-state concentrations of active metabolites (6-thioguaninenucleotides and 6-methylmercaptopurine nucleotides) in erythrocytes. A 2.2-fold decrease in myelosuppression, the primary endpoint, was observed in the gene-based-dose group using approximately 50% of the standard initial 6-MP dose (odds ratio, 0.26, 95% confidence interval, 0.11 to 0.64; p = 0.003). Patients in the gene-based-dose group had a significantly lower risk of developing thiopurine-induced myelosuppression and leukopenia (p = 0.015 and p = 0.022, respectively). No significant differences were observed in the secondary endpoints of the incidence of hepatotoxicity and steady-state concentrations of active metabolites in erythrocytes between the two groups. TPMT- and NUDT15-based dosing of 6-MP will significantly contribute toward further reducing the incidence of leukopenia in Chinese children with ALL. This trial is registered at www.clinicaltrial.gov as #NCT04228393.
Zhou, Yue; Wang, Li; Sun, Li-Rong; Zhang, Li; Wang, Hong-Mei; Liu, Xi-Ting; Yang, Fan; Wu, Ke-Liang; Liang, Yu-Li; Zhao, Bei-Bei; Zhuang, Yong; Fu, Jin-Qiu; Song, Chao; Li, Yun; Wang, Ling-Zhen; Xu, Hui-Juan; Gu, Yan; van den Anker, John; Ju, Xiu-Li; Zhu, Xiao-Fan; and Zhao, Wei, "Individualized use of 6-mercaptopurine in Chinese children with ALL: A multicenter randomized controlled trial" (2023). GW Authored Works. Paper 3343.