Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni
Cell reports methods
Schistosoma mansoni; gene safe harbor; human blood fluke; overlapping CRISPR target; reporter transgene
The identification and characterization of genomic safe harbor sites (GSHs) can facilitate consistent transgene activity with minimal disruption to the host cell genome. We combined computational genome annotation and chromatin structure analysis to predict the location of four GSHs in the human blood fluke, , a major infectious pathogen of the tropics. A transgene was introduced via CRISPR-Cas-assisted homology-directed repair into one of the GSHs in the egg of the parasite. Gene editing efficiencies of 24% and transgene-encoded fluorescence of 75% of gene-edited schistosome eggs were observed. The approach advances functional genomics for schistosomes by providing a tractable path for generating transgenics using homology-directed, repair-catalyzed transgene insertion. We also suggest that this work will serve as a roadmap for the development of similar approaches in helminths more broadly.
Ittiprasert, Wannaporn; Moescheid, Max F.; Chaparro, Cristian; Mann, Victoria H.; Quack, Thomas; Rodpai, Rutchanee; Miller, André; Wisitpongpun, Prapakorn; Buakaew, Watunyoo; Mentink-Kane, Margaret; Schmid, Sarah; Popratiloff, Anastas; Grevelding, Christoph G.; Grunau, Christoph; and Brindley, Paul J., "Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni" (2023). GW Authored Works. Paper 3035.
Microbiology, Immunology, and Tropical Medicine