A quantitative assessment of Staphylococcus aureus community carriage in Yuma, Arizona
The Journal of infectious diseases
Staphylococcus aureus; Culturing S. aureus; Quantitative carriage; SaQuant; carriage of S. aureus; molecular detection of S. aureus
Disease control relies on pathogen identification and understanding reservoirs. Staphylococcus aureus infection prevention is based upon decades of research on colonization and infection, but diminishing returns from mitigation efforts suggest significant knowledge gaps. Existing knowledge and mitigation protocols are founded upon culture-based detection, with almost no information about pathogen quantities. We employed a qPCR assay on samples from three body sites to characterize colonization more comprehensively than previous studies by describing both prevalence and pathogen quantity. We show a much higher overall prevalence (65.9%) than previously documented, with higher quantities and prevalence associated with the nares, non-Hispanic males (86.9%), and correlating with colonization in other body sites. These results suggest that research and clinical practices likely misclassify over half of colonized persons, limiting mitigation measures and their impact. This work begins the process of rebuilding foundational knowledge of S. aureus carriage with more accurate and wholistic approaches.
Russakoff, Benjamin; Wood, Colin; Lininger, Monica R.; Barger, Steven D.; Trotter, Robert T.; Maltinsky, Sara; Mbegbu, Mimi; Coyne, Briana; Yague, David Panisello; Kyman, Shari; Tucker-Morgan, Kara; Ceniceros, Kathya; Padilla, Cristina; Hurtado, Kevin; Menard, Ashley; Villa, Francisco; Wayment, Heidi A.; Hepp, Crystal; Furstenau, Tara; Fofanov, Viacheslav; Liu, Cindy M.; and Pearson, Talima R., "A quantitative assessment of Staphylococcus aureus community carriage in Yuma, Arizona" (2022). GW Authored Works. Paper 1983.
Environmental and Occupational Health