Development of an accessible and scalable qPCR assay for Monkeypox virus detection
The Journal of infectious diseases
Monkeypox; PCR; diagnostics; emerging infectious disease; global health; outbreak response; qPCR assay
During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard qPCR diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with Cq values of 17.46 to 35.59 and titer equivalents 8.01 × 106 to 2.45 × 100 PFU/mL. The detection limit was 3.59 PFU/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health labs to increase diagnostic capacity amid the growing MPX outbreak.
Porzucek, Abigail J.; Proctor, Abigale M.; Klinkhammer, Katharina E.; Tritsch, Sarah R.; Robertson, Molly A.; Bashor, Jonathan P.; Villani, Jack; Sepulveda, Jorge L.; and Mores, Christopher N., "Development of an accessible and scalable qPCR assay for Monkeypox virus detection" (2022). GW Authored Works. Paper 1780.