Title

An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Infection

Authors

Lakkhana Sadaow, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Rutchanee Rodpai, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Penchom Janwan, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Patcharaporn Boonroumkaew, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Oranuch Sanpool, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Tongjit Thanchomnang, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Hiroshi Yamasaki, Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Wannaporn Ittiprasert, Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA.
Victoria H. Mann, Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA.
Paul J. Brindley, Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, DC 20052, USA.
Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

Document Type

Journal Article

Publication Date

10-17-2022

Journal

Tropical medicine and infectious disease

Volume

7

Issue

10

DOI

10.3390/tropicalmed7100308

Keywords

IgG; clonorchiasis; immunochromatographic test kit; opisthorchiasis; point-of-care test; serodiagnosis; whole-blood sample

Abstract

Chronic human liver fluke infections caused by and can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS ( 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available.

Department

Microbiology, Immunology, and Tropical Medicine

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