School of Medicine and Health Sciences Poster Presentations

Title

Study of changes in the transcriptome of mouse bladder following bladder wall injection of Schistosoma haematobium eggs

Poster Number

277

Document Type

Poster

Status

Postdoc

Abstract Category

Immunology/Infectious Diseases

Keywords

schistosoma, haematobium, eggs, transcriptome, bladder

Publication Date

Spring 2018

Abstract

Background: Infection with Schistosoma haematobium leads to urogenital schistosomiasis (UGS), which afflicts over 100 million people. UGS can cause hematuria, calcification of the bladder, and increased risk of secondary infections by bacteria or viruses, and it is also linked to bladder cancer. Previous studies have used a mouse model that involves injection of S. haematobium eggs into the bladder wall to examine the effect of parasite eggs on host bladder biology, and they have identified changes in genome-wide methylation, as well as changes in the transcript level of some genes by microarray analysis. Here, we perform RNA-Seq on egg injected bladders to expand the detection of changes in gene transcript level to the scale of the whole transcriptome. Methods: We followed a combined infection/injection animal model in which female BALB/c mice were infected with S. haematobium cercariae and then, after 5 weeks, administered either parasite eggs or hamster liver extract by bladder wall injection. Another group of control mice did not receive surgery. RNA-Seq was performed on the RNA isolated from the bladders 4 days after bladder wall injection. Results/Conclusions: RNA-Seq analysis of egg-injected bladders and controls will reveal host gene pathways activated by the presence of S. haematobium eggs. Of particular interest are urothelial cell-related genes, including cancer pathways, differentially expressed genes associated with infiltrating and resident leukocytes, and fibrosis-related gene pathways. RNA-Seq analysis of schistosome-specific genes highlight the challenges of performing the parasite side of dual RNA-Seq in the setting of low amounts of parasite RNA relative to host RNA.

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Study of changes in the transcriptome of mouse bladder following bladder wall injection of Schistosoma haematobium eggs

Background: Infection with Schistosoma haematobium leads to urogenital schistosomiasis (UGS), which afflicts over 100 million people. UGS can cause hematuria, calcification of the bladder, and increased risk of secondary infections by bacteria or viruses, and it is also linked to bladder cancer. Previous studies have used a mouse model that involves injection of S. haematobium eggs into the bladder wall to examine the effect of parasite eggs on host bladder biology, and they have identified changes in genome-wide methylation, as well as changes in the transcript level of some genes by microarray analysis. Here, we perform RNA-Seq on egg injected bladders to expand the detection of changes in gene transcript level to the scale of the whole transcriptome. Methods: We followed a combined infection/injection animal model in which female BALB/c mice were infected with S. haematobium cercariae and then, after 5 weeks, administered either parasite eggs or hamster liver extract by bladder wall injection. Another group of control mice did not receive surgery. RNA-Seq was performed on the RNA isolated from the bladders 4 days after bladder wall injection. Results/Conclusions: RNA-Seq analysis of egg-injected bladders and controls will reveal host gene pathways activated by the presence of S. haematobium eggs. Of particular interest are urothelial cell-related genes, including cancer pathways, differentially expressed genes associated with infiltrating and resident leukocytes, and fibrosis-related gene pathways. RNA-Seq analysis of schistosome-specific genes highlight the challenges of performing the parasite side of dual RNA-Seq in the setting of low amounts of parasite RNA relative to host RNA.