Milken Institute School of Public Health Poster Presentations (Marvin Center & Video)

Title

Predicting Oxidative Stress Potential Through Spectroscopic Methods

Poster Number

49

Document Type

Poster

Status

Undergraduate Student

Abstract Category

Environmental and Occupational Health

Keywords

Toxicology, green chemistry, environmental health

Publication Date

Spring 2018

Abstract

Rational design of safer chemicals is of the upmost importance. The potential of chemicals to cause oxidative stress, the generation of free radicals, must be investigated. Oxidative stress is chosen as the ultimate endpoint because it encompasses critical toxicological pathways for many environmentally adverse chemicals and has been linked to cancer, cardiovascular disease, Alzheimer’s disease, and AIDS. In response to high levels of free radicals, the Nrf2 pathway will signal the release of glutathione to react with the radical species. It is understood that chemicals which react with a glutathione model likely cause oxidative stress). The glutathione model is the molecule 5-(Dimethylamino)-N-(2-mercaptoethyl)naphthalene-1-sulfonamide (DCYA). The glutathione model will be synthesized using a procedure found in Chemical Research in Toxicology (Chittiboyina, et al). Chemicals with mutagenic properties will be reacted with DYCA to probe oxidative stress potential. The reaction will be monitored by NMR spectroscopy to quantify the depletion of the chemical. NMR spectroscopy provides information on the reactivity of the chemicals and the quantifies the structure of the final adduct.

Multiple chemicals have been successfully tested with the model thiol. The products of the reaction have been monitored and structurally investigated through NMR spectroscopy. The above procedure has yielded an efficient method to determine the oxidative stress potential of chemicals.

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Creative Commons License
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Predicting Oxidative Stress Potential Through Spectroscopic Methods

Rational design of safer chemicals is of the upmost importance. The potential of chemicals to cause oxidative stress, the generation of free radicals, must be investigated. Oxidative stress is chosen as the ultimate endpoint because it encompasses critical toxicological pathways for many environmentally adverse chemicals and has been linked to cancer, cardiovascular disease, Alzheimer’s disease, and AIDS. In response to high levels of free radicals, the Nrf2 pathway will signal the release of glutathione to react with the radical species. It is understood that chemicals which react with a glutathione model likely cause oxidative stress). The glutathione model is the molecule 5-(Dimethylamino)-N-(2-mercaptoethyl)naphthalene-1-sulfonamide (DCYA). The glutathione model will be synthesized using a procedure found in Chemical Research in Toxicology (Chittiboyina, et al). Chemicals with mutagenic properties will be reacted with DYCA to probe oxidative stress potential. The reaction will be monitored by NMR spectroscopy to quantify the depletion of the chemical. NMR spectroscopy provides information on the reactivity of the chemicals and the quantifies the structure of the final adduct.

Multiple chemicals have been successfully tested with the model thiol. The products of the reaction have been monitored and structurally investigated through NMR spectroscopy. The above procedure has yielded an efficient method to determine the oxidative stress potential of chemicals.