Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium

Document Type

Journal Article

Publication Date

2014

Journal

Journal of Tissue Engineering and Regenerative Medicine

Abstract

Improvements in ex vivo generation of enucleated red blood cells are being sought for erythroid biology research, toward the ultimate goal of erythrocyte engineering for clinical use. Based upon the high levels of iron-saturated transferrin in plasma serum, it was hypothesized that terminal differentiation in serum-free media may be highly dependent on the concentration of iron. Here adult human CD34+ cells were cultured in a serum-free medium containing dosed levels of iron-saturated transferrin (holo-Tf, 0.1–1.0 mg/ml). Iron in the culture medium was reduced, but not depleted, with erythroblast differentiation into haemoglobinized cells. At the lowest holo-Tf dose (0.1 mg/ml), terminal differentiation was significantly reduced and the majority of the cells underwent apoptotic death. Cell survival, differentiation and enucleation were enhanced as the holo-Tf dose increased. These data suggest that adequate holo-Tf dosing is critical for terminal differentiation and enucleation of human erythroblasts generated ex vivo in serum-free culture conditions

Comments

This article is a U.S. Government work and is in the public domain in the USA.

Originally published in Wiley, Journal of Tissue Engineering and Regenerative Medicine.

Creative Commons License

Creative Commons License
This work is free of known copyright restrictions.

Peer Reviewed

1

Open Access

1

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