Document Type
Journal Article
Publication Date
2016
Journal
Journal of Cancer
Volume
7
Issue
11
Inclusive Pages
1472-1480
DOI
10.7150/jca.14844
Abstract
Increased expression of progesterone receptor (PR) has been reported in gastric cancer (GC). We have previously identified a functional T889C point mutation in DNA polymerase beta (POLB), a DNA repair gene in GC. To provide a detailed analysis of molecular changes associated with the mutation, human cDNA microarrays focusing on 18 signal transduction pathways were used to analyze differential gene expression profiles between GC tissues with T889C mutant in POLB gene and those with wild type. Among the differentially expressed genes, notably, PR was one of the significantly up-regulated genes in T889C mutant POLB tissues, which were subsequently confirmed in POLB gene transfected AGS cell line. Interestingly, patients with T889C mutation and PR positivity were associated with higher incidence of intraperitoneal metastasis (IM). In vitro studies indicate that PR expression was upregulated in AGS cell line when transfected with T889C mutant expression vector. Cotransfection of T889C mutant allele and PR gene induced cell migration in the cell line. These data demonstrated that T889C mutation-associated PR overexpression results in increased IM. Therefore, T889C mutation-associated PR overexpression may serve as a biomarker for an adverse prognosis for human GC.
APA Citation
Tan, X., Wu, X., Ren, S., Wang, H., Alshenawy, W., Li, W., Cui, J., Luo, G., Siegel, R. S., Fu, S. W., & Lu, Y. (2016). A Point Mutation in DNA Polymerase β (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer. Journal of Cancer, 7 (11). http://dx.doi.org/10.7150/jca.14844
Peer Reviewed
1
Open Access
1
Included in
Cancer Biology Commons, Genetics and Genomics Commons, Medicine and Health Sciences Commons
Comments
Reproduced with permission of Ivyspring International Publisher. Journal of Cancer
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