Title

Nociceptin inhibits γ-aminobutyric acidergic inputs to cardiac parasympathetic neurons in the nucleus ambiguus

Document Type

Journal Article

Publication Date

1-12-2002

Journal

Journal of Pharmacology and Experimental Therapeutics

Volume

300

Issue

1

DOI

10.1124/jpet.300.1.78

Abstract

Studies have shown that nociceptin, the endogenous ligand for the opioid receptor-like receptor (ORL1), modulates central control of cardiovascular activity. The nucleus ambiguus, an area containing cardiac parasympathetic neurons, contains both ORL1 receptors and neurons that contain nociceptin itself. Although previous work has shown that nociceptin acts to increase parasympathetic outflow to the heart, the mechanisms by which this is achieved are unknown. In the present study, the effects of nociceptin on spontaneous γ-aminobutyric acidergic (GABAergic) input to cardiac parasympathetic neurons (IPSCs) was examined. At 100 μM, nociceptin inhibited both the frequency (-35.6%) and the amplitude (-49.5%) of spontaneous GABAergic IPSCs in cardiac vagal neurons. Nociceptin also caused a novel postsynaptic inhibition of the responses evoked by exogenous application of GABA. These results indicate that nociceptin acts both on neurons precedent to cardiovascular neurons to decrease the activity of GABAergic neurons that synapse upon cardiovascular neurons and directly, inhibiting the postsynaptic currents evoked by GABA. This inhibition by nociceptin would increase parasympathetic outflow to the heart, thus providing a possible mechanism for nociceptin-induced bradycardia.

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