Document Type

Journal Article

Publication Date



Journal of Biological Chemistry


Volume 287, Issue 36

Inclusive Pages



Mitochondria; Muscle--metabolism; Muscle Fibers; Skeletal--metabolism; Muscle Proteins--metabolism; Proteomics; Sarcolemma--metabolism; Wound Healing--physiology


Skeletal muscles are proficient at healing from a variety of injuries. Healing occurs in two phases, early and late phase. Early phase involves healing the injured sarcolemma and restricting the spread of damage to the injured myofiber. Late phase of healing occurs a few days postinjury and involves interaction of injured myofibers with regenerative and inflammatory cells. Of the two phases, cellular and molecular processes involved in the early phase of healing are poorly understood. We have implemented an improved sarcolemmal proteomics approach together with in vivo labeling of proteins with modified amino acids in mice to study acute changes in the sarcolemmal proteome in early phase of myofiber injury. We find that a notable early phase response to muscle injury is an increased association of mitochondria with the injured sarcolemma. Real-time imaging of live myofibers during injury demonstrated that the increased association of mitochondria with the injured sarcolemma involves translocation of mitochondria to the site of injury, a response that is lacking in cultured myoblasts. Inhibiting mitochondrial function at the time of injury inhibited healing of the injured myofibers. This identifies a novel role of mitochondria in the early phase of healing injured myofibers.


This research was originally published in Journal of Biological Chemistry. Nimisha Sharma, Sushma Medikayala, Aurelia Defour, Sree Rayavarapu, Kristy J. Brown, et al. Use of quantitative membrane proteomics identifies a novel role of mitochondria in healing injured muscles. Journal of Biological Chemistry. 2012; 287:30455-30467. © the American Society for Biochemistry and Molecular Biology.

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