Title

Elucidating the role of lipid rafts on g protein-coupled receptor function in the mouse kidney: An in vivo approach

Document Type

Journal Article

Publication Date

1-1-2021

Journal

Methods in Molecular Biology

Volume

2187

DOI

10.1007/978-1-0716-0814-2_10

Keywords

Kidney; Lipid raft; Methyl-β-cyclodextrin; Mini osmotic pump; Mouse; α-Cyclodextrin

Abstract

© 2021, Springer Science+Business Media, LLC, part of Springer Nature. Numerous G protein-coupled receptors (GPCRs) and GPCR-signaling molecules reside in lipid rafts and thus, are inherently regulated in these microdomains. However, the limitations of current methods to investigate lipid raft biology and GPCR activity in situ have hindered the complete understanding of the molecular underpinnings of GPCR trafficking and signaling, especially in the whole organism. This book chapter details an innovative in vivo approach to study the crucial role of lipid rafts on the workings of GPCRs in the mouse kidney. This protocol involves the use of a modified mini osmotic pump to deliver an agent that selectively disrupts the lipid raft in the kidney.

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