Biochemical and Biophysical Research Communications
Animals; Cells, Cultured; Dependovirus; Gene Expression Regulation; Gene Transfer Techniques; Genes, Viral; Genetic Therapy; Genetic Vectors; Mice; Mice, Inbred C57BL; Nephrons
AAV9 vector provides efficient gene transfer in all segments of the renal nephron, with minimum expression in non-renal cells, when administered retrogradely via the ureter. It is important to restrict the transgene expression to the desired cell type within the kidney, so that the physiological endpoints represent the function of the transgene expressed in that specific cell type within kidney. We hypothesized that segment-specific gene expression within the kidney can be accomplished using the highly efficient AAV9 vectors carrying the promoters of genes that are expressed exclusively in the desired segment of the nephron in combination with administration by retrograde infusion into the kidney via the ureter. We constructed AAV vectors carrying eGFP under the control of: kidney-specific cadherin (KSPC) gene promoter for expression in the entire nephron; Na
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Asico, L., Cuevas, S., Ma, X., Jose, P., Armando, I., & Konkalmatt, P. (2018). Nephron segment-specific gene expression using AAV vectors.. Biochemical and Biophysical Research Communications, 497 (1). http://dx.doi.org/10.1016/j.bbrc.2018.01.169