Evaluation of the Performance of Portable Hemoglobinometers at Measuring Hemoglobin and Detecting Anemia in a Periurban Pediatric Population in Lima, Peru
Authors
Zach Silverstein, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland.
Alicia Alva Mantari, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Kimberly Breglio, Department of Dermatology, Duke University Hospital, Durham, North Carolina.
Monica J. Pajuelo, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Gladys Valdiviezo, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Sandra Cruz, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Pamela Castañeda, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Jessica D. Rothstein, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland.
Lucia Huaman-Fernandez, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Lilia Cabrera, Asociación Benéfica PRISMA, Lima, Peru.
Mario Salguedo, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Franklin Barrientos, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Bryan Saldivar-Espinoza, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Dennis Núñez-Fernández, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Robert H. Gilman, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland.
Patricia Sheen, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Avid Roman-Gonzalez, Image Processing Research Laboratory, Universidad de Ciencias y Humanidades, Lima, Peru.
Sassan Noazin, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland.
Mirko Zimic, Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e ingeniería, Universidad Peruana Cayetano Heredia.
Document Type
Journal Article
Publication Date
12-10-2024
Journal
The American journal of tropical medicine and hygiene
DOI
10.4269/ajtmh.24-0494
Abstract
As many as one in three people worldwide have anemia, with young children at increased risk of both disease and complications. In settings without clinical laboratories, portable hemoglobinometers serve important roles in diagnosing anemia and estimating prevalence. Here, we assess the validity of two such point-of-care devices-the HemoCue Hb201 and the HemoCue Hb301-relative to the international reference standard, the cyanmethemoglobin method. In total, 428 children ages 6-60 months were recruited at health posts in Lima, Peru, and venous and capillary blood samples were collected from each participant. Venous blood was assessed with the cyanmethemoglobin method, whereas capillary blood was assessed using the Hb201 and the Hb301; 16.1% of participants were found to have anemia using the cyanmethemoglobin method. Both the Hb201 (43.7%) and the Hb301 (20.6%) overestimated this prevalence, with the former reaching statistical significance (P <0.0001 and P = 0.11, respectively). Both devices also tended to underestimate hemoglobin concentration, with the Hb201 (mean difference = -0.99 g/dL; percentage error = -8.1%) being appreciably less accurate than the Hb301 (mean difference = -0.35 g/dL; percentage error = -2.7%). Areas under the curve for the Hb201 (0.92) and the Hb301 (0.93) were statistically similar (P = 0.28); however, the Hb201 incorrectly classified 29.4% of participants compared with 11.0% for the Hb301. Both devices had more false positives than false negatives. In conclusion, the Hb301 was found to be significantly more accurate than the Hb201 at measuring hemoglobin, diagnosing anemia, and estimating anemia prevalence.
APA Citation
Silverstein, Zach; Alva Mantari, Alicia; Breglio, Kimberly; Pajuelo, Monica J.; Valdiviezo, Gladys; Cruz, Sandra; Castañeda, Pamela; Rothstein, Jessica D.; Huaman-Fernandez, Lucia; Cabrera, Lilia; Salguedo, Mario; Barrientos, Franklin; Saldivar-Espinoza, Bryan; Núñez-Fernández, Dennis; Gilman, Robert H.; Sheen, Patricia; Roman-Gonzalez, Avid; Noazin, Sassan; and Zimic, Mirko, "Evaluation of the Performance of Portable Hemoglobinometers at Measuring Hemoglobin and Detecting Anemia in a Periurban Pediatric Population in Lima, Peru" (2024). GW Authored Works. Paper 6174.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/6174
