School of Medicine and Health Sciences Poster Presentations

Title

The Theorized Effects of Cold Atmospheric Plasma on the Membrane Potential Across the Glioma Cell Membrane

Document Type

Poster

Keywords

Oncology; cancer; Glioblastoma multiforme; cold atmospheric plasma

Publication Date

Spring 2017

Abstract

TITLE: The Theorized Effects of Cold Atmospheric Plasma on the Membrane Potential Across the Glioma Cell Membrane

BACKGROUND: Cold atmospheric plasma (CAP) is a novel but promising potential therapy for specifically targeting neoplastic cells. It has been shown to selectively induce apoptosis in certain types of cancer cells to a much larger extent than healthy cells via generation of reactive oxygen and nitrogen species, which damage DNA. The mechanism by which CAP preferentially targets cancer cells over normal cells is unknown at this time.

HYPOTHESIS: We hypothesize that treating glioma cells with CAP will transiently hyperpolarize the cell membrane, pausing cells in the G2 phase of the cell cycle and preventing progression to mitosis in glioma cells at a higher rate than healthy cells. We believe that this will be subsequently followed by cell membrane depolarization and apoptosis in glioma cells at a higher rate than healthy glial cells. We believe that the hyperpolarization differences seen between cell lines will be due to increased aquaporin expression on glioma cell membranes and that the depolarization differences will be due to the increased inward calcium channel expression on the glioma cell membrane, which can be directly activated by CAP.

PROPOSED METHODS: We anticipate measuring glioma cell membrane potentials before and after CAP treatment and comparing our results to glial cell controls over a specified time period. We plan to measure the potentials using a fluorescent membrane potential-measuring dye, which can be visualized by microscopy. Another possibility would be to directly measure individual cell’s membrane potentials and their changes using a microelectrode inserted directly into an individual cell.

RESULTS/CONCLUSION: Upon conclusion of the project.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Open Access

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Comments

Poster to be presented at GW Annual Research Days 2017.

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The Theorized Effects of Cold Atmospheric Plasma on the Membrane Potential Across the Glioma Cell Membrane

TITLE: The Theorized Effects of Cold Atmospheric Plasma on the Membrane Potential Across the Glioma Cell Membrane

BACKGROUND: Cold atmospheric plasma (CAP) is a novel but promising potential therapy for specifically targeting neoplastic cells. It has been shown to selectively induce apoptosis in certain types of cancer cells to a much larger extent than healthy cells via generation of reactive oxygen and nitrogen species, which damage DNA. The mechanism by which CAP preferentially targets cancer cells over normal cells is unknown at this time.

HYPOTHESIS: We hypothesize that treating glioma cells with CAP will transiently hyperpolarize the cell membrane, pausing cells in the G2 phase of the cell cycle and preventing progression to mitosis in glioma cells at a higher rate than healthy cells. We believe that this will be subsequently followed by cell membrane depolarization and apoptosis in glioma cells at a higher rate than healthy glial cells. We believe that the hyperpolarization differences seen between cell lines will be due to increased aquaporin expression on glioma cell membranes and that the depolarization differences will be due to the increased inward calcium channel expression on the glioma cell membrane, which can be directly activated by CAP.

PROPOSED METHODS: We anticipate measuring glioma cell membrane potentials before and after CAP treatment and comparing our results to glial cell controls over a specified time period. We plan to measure the potentials using a fluorescent membrane potential-measuring dye, which can be visualized by microscopy. Another possibility would be to directly measure individual cell’s membrane potentials and their changes using a microelectrode inserted directly into an individual cell.

RESULTS/CONCLUSION: Upon conclusion of the project.