Department of Biomedical Engineering Posters and Presentations

Title

Effects of ultrasound in presence of microbubbles for cartilage and bone tissue regeneration in 3D printed scaffolds

Document Type

Poster

Keywords

Ultrasound, Microbubbles, Tissue Engineering

Publication Date

4-2017

Abstract

Gas-filled microbubbles encapsulated with lipids and other surfactants are highly responsive to ultrasound, which has led to their effective role as ultrasound contrast agents (UCA). In this study, for the first time, we used lipid-coated microbubbles (MB) and lipid and nano-hydroxyapetite (n-HA) coated MB prepared in-house to better harness the beneficial effects of ultrasound stimulation on proliferation and chondrogenic and osteogenic differentiation of human mesenchymal stem cells (MSCs) within novel 3D printed poly (ethylene glycol) diacrylate (PEG-DA) hydrogel scaffolds with and without embedded n-HA. A significant increase in cell number (p<0.001) was observed with low intensity pulsed ultrasound (LIPUS) treatment in the presence of 0.5 % (v/v) MB and lipid and n-HA coated MB after 1, 3 and 5 days of culture in scaffolds with and without n-HA. MSC proliferation increased by 20% with LIPUS, 37% with LIPUS and MB, and 43% with LIPUS and lipid and n-HA coated MB for one day studies. MSC proliferation was also enhanced up to 40% after 5 days of culture in presence of MB and LIPUS while this value was only 18% when excited with LIPUS alone in scaffolds not embedded with n-HA. Our 3-week chondrogenic differentiation results demonstrated that combining LIPUS with MB significantly enhanced both Glycosaminoglycan (GAG) and type II collagen production. Therefore, integrating LIPUS and MB appears to be a promising strategy for enhanced MSC growth and chondrogenic and osteogenic differentiation for potential tissue engineering and regeneration therapies.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Open Access

1

Comments

To be presented at GW Annual Research Days 2017.

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Effects of ultrasound in presence of microbubbles for cartilage and bone tissue regeneration in 3D printed scaffolds

Gas-filled microbubbles encapsulated with lipids and other surfactants are highly responsive to ultrasound, which has led to their effective role as ultrasound contrast agents (UCA). In this study, for the first time, we used lipid-coated microbubbles (MB) and lipid and nano-hydroxyapetite (n-HA) coated MB prepared in-house to better harness the beneficial effects of ultrasound stimulation on proliferation and chondrogenic and osteogenic differentiation of human mesenchymal stem cells (MSCs) within novel 3D printed poly (ethylene glycol) diacrylate (PEG-DA) hydrogel scaffolds with and without embedded n-HA. A significant increase in cell number (p<0.001) was observed with low intensity pulsed ultrasound (LIPUS) treatment in the presence of 0.5 % (v/v) MB and lipid and n-HA coated MB after 1, 3 and 5 days of culture in scaffolds with and without n-HA. MSC proliferation increased by 20% with LIPUS, 37% with LIPUS and MB, and 43% with LIPUS and lipid and n-HA coated MB for one day studies. MSC proliferation was also enhanced up to 40% after 5 days of culture in presence of MB and LIPUS while this value was only 18% when excited with LIPUS alone in scaffolds not embedded with n-HA. Our 3-week chondrogenic differentiation results demonstrated that combining LIPUS with MB significantly enhanced both Glycosaminoglycan (GAG) and type II collagen production. Therefore, integrating LIPUS and MB appears to be a promising strategy for enhanced MSC growth and chondrogenic and osteogenic differentiation for potential tissue engineering and regeneration therapies.